Difference between revisions of "Part:BBa K774001:Experience"
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| − | [[Image:NRPMBPhoto.jpg | right | | + | [[Image:NRPMBPhoto.jpg | right | thumbnail | 200px | Pellets of ''E. coli'' transformed by this part ligated to BBa_E0420 (an RBS and CFP reporter) and grown in media with added concentrations of potassium nitrate. Going from left to right in concentrations of potassium nitrate: 10 mM, 50 mM, 100 mM and 0 mM]] |
This part was ligated with [https://parts.igem.org/wiki/index.php/Part:BBa_E0420 BBa_E0420] to give a ribosome binding site (RBS) and cyan fluorescent protein (CFP) reporter. ''Escherichia coli'' was then transformed by the part ligated with BBa_E0420 and grown in media also containing potassium nitrate (as a source of nitrates in order to induce promoter activity) at concentrations of 0 mM, 10 mM, 50 mM and 100 mM. The ''E. coli'' was grown for 6 hours and then added to eppendorf tubes and spun down in a centrifuge in order to produce a pellet. The four samples were then viewed under a UV box to assess for fluorescence; as the accompanying photograph shows the sample at 0 mM potassium nitrate did not fluoresce, however those at 10, 50 and 100 mM potassium nitrate did fluoresce. They also appeared to fluoresce at the same strength, suggesting that 10 mM was equal to or above the maximum sensitivity level of this part. | This part was ligated with [https://parts.igem.org/wiki/index.php/Part:BBa_E0420 BBa_E0420] to give a ribosome binding site (RBS) and cyan fluorescent protein (CFP) reporter. ''Escherichia coli'' was then transformed by the part ligated with BBa_E0420 and grown in media also containing potassium nitrate (as a source of nitrates in order to induce promoter activity) at concentrations of 0 mM, 10 mM, 50 mM and 100 mM. The ''E. coli'' was grown for 6 hours and then added to eppendorf tubes and spun down in a centrifuge in order to produce a pellet. The four samples were then viewed under a UV box to assess for fluorescence; as the accompanying photograph shows the sample at 0 mM potassium nitrate did not fluoresce, however those at 10, 50 and 100 mM potassium nitrate did fluoresce. They also appeared to fluoresce at the same strength, suggesting that 10 mM was equal to or above the maximum sensitivity level of this part. | ||
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Revision as of 19:09, 16 September 2012
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Applications of BBa_K774001
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No review score entered. RussellGritton |
This part was ligated with BBa_E0420 to give a ribosome binding site (RBS) and cyan fluorescent protein (CFP) reporter. Escherichia coli was then transformed by the part ligated with BBa_E0420 and grown in media also containing potassium nitrate (as a source of nitrates in order to induce promoter activity) at concentrations of 0 mM, 10 mM, 50 mM and 100 mM. The E. coli was grown for 6 hours and then added to eppendorf tubes and spun down in a centrifuge in order to produce a pellet. The four samples were then viewed under a UV box to assess for fluorescence; as the accompanying photograph shows the sample at 0 mM potassium nitrate did not fluoresce, however those at 10, 50 and 100 mM potassium nitrate did fluoresce. They also appeared to fluoresce at the same strength, suggesting that 10 mM was equal to or above the maximum sensitivity level of this part. |
