Difference between revisions of "Part:BBa K784020:Design"

 
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===Design Notes===
 
===Design Notes===
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Hila, update this.
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This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation. <br>
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The main goal with the genome deviation to eight fragments was to avoid putting regulatory elements with their regulated sequences. By doing so, we can prevent the expression of harmful and lytic associated proteins to the host bacteria. Moreover, due to the phage lambda genome organization we could divide the phages genome while each part contains several proteins which are in functional correlation with each other. <br>
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===Source===
 
===Source===

Latest revision as of 16:03, 21 September 2012

Phage Lambda fragment 6 - regulation


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3822
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1500
    Illegal PstI site found at 3822
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2532
    Illegal BglII site found at 4924
    Illegal BglII site found at 5575
    Illegal BglII site found at 5635
    Illegal BamHI site found at 1320
    Illegal XhoI site found at 319
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3822
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3822
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1148
    Illegal SapI.rc site found at 1621


Design Notes

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
The main goal with the genome deviation to eight fragments was to avoid putting regulatory elements with their regulated sequences. By doing so, we can prevent the expression of harmful and lytic associated proteins to the host bacteria. Moreover, due to the phage lambda genome organization we could divide the phages genome while each part contains several proteins which are in functional correlation with each other.

Source

http://www.ncbi.nlm.nih.gov/nuccore/NC_001416.1

The physical DNA was obtained from NEB - http://www.neb.com/nebecomm/products/productn3011.asp


References