Difference between revisions of "Part:BBa K801999:Design"
VolkerMorath (Talk | contribs) (→Design Notes) |
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<!--*Part was designed in RFC10/RFC23/RFC25--> | <!--*Part was designed in RFC10/RFC23/RFC25--> | ||
<!--*Mutation C889G to delete XbaI restriction site--> | <!--*Mutation C889G to delete XbaI restriction site--> | ||
− | <!--* | + | <!--*Truncation upstream/downstream compared to template in order to ???--> |
− | <!--* | + | |
+ | '''Quality control measures:'''<br> | ||
+ | <!--*Test-digestion using ?enzyme1? & ?enzyme2?/Not yet performed--> | ||
+ | <!--*Sequencing using primer ?primer_name?/Not yet sequenced--> | ||
'''Backbone:'''<br> | '''Backbone:'''<br> | ||
− | <!--*Backbone name: ?backbone_name | + | <!--*Backbone name: pSB1C3'/?backbone_name?--> |
− | + | <!--*Resistance: Amp/Cp/Kan/--> | |
− | <!--*Resistance: | + | |
<!--*Copynumber: low/medium/high--> | <!--*Copynumber: low/medium/high--> | ||
− | |||
− | |||
− | |||
− | |||
'''Protein coding:'''<br> | '''Protein coding:'''<br> | ||
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<!--*The protein has the amino acid replacements ???99??? to ???99???.--> | <!--*The protein has the amino acid replacements ???99??? to ???99???.--> | ||
<!--*The protein encoded is posttranslationally modified by ???.--> | <!--*The protein encoded is posttranslationally modified by ???.--> | ||
− | <!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/ | + | <!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other--> |
'''Enzymatic activity:''' | '''Enzymatic activity:''' | ||
<!--none/EC-number ?.?.?.?--> | <!--none/EC-number ?.?.?.?--> | ||
− | '''Cytotoxicity:''' | + | '''Cytotoxicity:'''<br> |
<!--none/not known/cytotoxic for ''organism name''--> | <!--none/not known/cytotoxic for ''organism name''--> | ||
+ | |||
+ | ''Safety notes'''<br> | ||
+ | <!--Known and anticipated sefety issues: none/health_risk/environmental_risk--> | ||
+ | <!--Known and anticipated security issues.--> | ||
'''Intellectual property:''' | '''Intellectual property:''' | ||
− | <!-- | + | <!--Information on patent situation.--> |
+ | <!--Intellectual property claims made by the authors.--> | ||
+ | |||
+ | '''Contact information:''' | ||
+ | <!--https://igem.org/User_Information.cgi?user_id=????/email--> | ||
===Source=== | ===Source=== | ||
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<!--*Genesequence derived from ''?organism_name?''--> | <!--*Genesequence derived from ''?organism_name?''--> | ||
<!--*Codonoptimized for ''?organism_name?''--> | <!--*Codonoptimized for ''?organism_name?''--> | ||
+ | <!--*Designed for the following Chassis: ''?organism-name?''--> | ||
+ | <!--*Statement about functionality in other chassis.--> | ||
+ | |||
===References=== | ===References=== |
Revision as of 23:39, 21 September 2012
Test page for standardized BioBrick part descriptions
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Keywords:
Abbreviations:
Design Notes
Other versions of this BioBrick:
Cloning details:
Quality control measures:
Backbone:
Protein coding:
Enzymatic activity:
Cytotoxicity:
Safety notes'
Intellectual property:
Contact information:
Source
Source:
Organism:
References
Literature references:
Sequence references:
Structure reference:
Test page for standardized BioBrick part descriptions
Keywords:
fluorescent, reporter, chromophore, luminescence, bioluminescence, photoprotein
Used abbreviations:
- GFP = Green Fluorescent Protein
Design Notes
Other versions of this BioBrick:
- The BioBrick BBa_I757008 encodes a yellow fluorescent protein (mVenus) derived from GFP
- The BioBrick BBa_I757008 encodes GFP in RFC25 for protein fusions
Cloning details:
- Part was designed in RFC10
- Mutation G381A to delete XbaI restriction site
- The correctness of the part was checked by sequencing
Protein coding:
- Green Fluorescent Protein [Nucleotide 1 to 714]
- The protein has the amino acid replacements Ser65Thr in order to increase fluorescence, photostability and to shift the major excitation peak to 488 nm. (see Heim et al., 1995)
- The protein encoded is posttranslationally modified by a cross-link between Ser65 and Gly67 to form the chromophore.
Enzymatic activity: none
Cytotoxicity: none
Source
Source:
- Amplified from plasmid: pSB1C3-GFP-generator, provided by Osamu Shimomura, Boston University School of Medicine, USA
Forward Primer:
5'- ATGATGATGATG - 3'
Reverse Primer:
5'- ATGATGATGATG - 3'
Organism:
- Sequence derived from Aequorea victoria
- Codon optimized for Escherichia coli
References
Literature references:
- [http://www.ncbi.nlm.nih.gov/pubmed/20010584 Pubmed: Prasher, 1995: Using GFP to see the light.(Review)]
- [http://www.ncbi.nlm.nih.gov/pubmed/7854443 Pubmed: Heim, 1995: Improved green fluorescence.(Reference for Chromophore)]
Sequence references:
- [http://www.ncbi.nlm.nih.gov/nuccore/X83959.1 GenBank: A.victoria mRNA for green fluorescent protein (ID:gfp1)]
- [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR011584 Interpro: Green fluorescent protein-related ]
- [http://www.uniprot.org/uniprot/P42212 Uniprot: Green fluorescent protein]
- [http://pfam.sanger.ac.uk/family/PF01353 Pfam: Green fluorescent protein]
Structure reference:
- [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EMA PDB: Green Fluorescent Protein from Aequorea victoria]