Difference between revisions of "Part:BBa K733009"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K733009 short</partinfo> | <partinfo>BBa_K733009 short</partinfo> | ||
− | + | pTms is a promoter which proves to be functional in both ''E. coli'' and ''B. subilis''. R.P.U standard is advocated by part registry to represent the efficiency of a constitutive promoter.(Kelly et al., 2009) We intend to characterize promoter pTms in ''E. coli''. Using ''E. coli'' as our initial characterization chassis may prove to be more valuable for most of iGEM participants, because ''E. coli'' is frequently used by iGEM participants. | |
+ | [https://parts.igem.org/Part:BBa_K733001 pTms promoter] | ||
+ | '''Link with our characterization page.''' | ||
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<partinfo>BBa_K733009 parameters</partinfo> | <partinfo>BBa_K733009 parameters</partinfo> | ||
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+ | ==Reference== | ||
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+ | Kelly J, Rubin A, Davis J, Ajo-Franklin C, Cumbers J, Czar M, de Mora K, Glieberman A, Monie D, Endy D: Measuring the activity of BioBrick promoters using an in vivo reference standard. ''Journal of Biological Engineering'' 2009, 3:4. |
Revision as of 17:18, 22 September 2012
Ptms+BBa_E0240: Ptms+RBS+GFP+Double
pTms is a promoter which proves to be functional in both E. coli and B. subilis. R.P.U standard is advocated by part registry to represent the efficiency of a constitutive promoter.(Kelly et al., 2009) We intend to characterize promoter pTms in E. coli. Using E. coli as our initial characterization chassis may prove to be more valuable for most of iGEM participants, because E. coli is frequently used by iGEM participants. pTms promoter Link with our characterization page.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 736
Reference
Kelly J, Rubin A, Davis J, Ajo-Franklin C, Cumbers J, Czar M, de Mora K, Glieberman A, Monie D, Endy D: Measuring the activity of BioBrick promoters using an in vivo reference standard. Journal of Biological Engineering 2009, 3:4.