Difference between revisions of "Part:BBa K929001:Design"
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<partinfo>BBa_K929001 short</partinfo> | <partinfo>BBa_K929001 short</partinfo> | ||
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GCCTGCAGCGGCCGCTACTAGTATTAACCGGTGGGCAAAAGGATGCGCCGAAGC | GCCTGCAGCGGCCGCTACTAGTATTAACCGGTGGGCAAAAGGATGCGCCGAAGC | ||
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===Source=== | ===Source=== | ||
Revision as of 06:21, 17 September 2012
AID without NES, with NLS and Kozak sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 579
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 108
Illegal SapI site found at 209
Design Notes
Primer (forward) with XbaI recognition site, kozak consensus sequence, NLS: ATCTAGAGCCGCCACCATGGGACCCAAGAAGAGGAAGGTGATGGACAGCCTCTTGATGAACCGGAGG
Primer (reverse, complement)with AgeI, SpeI and PstI recognition site: GCCTGCAGCGGCCGCTACTAGTATTAACCGGTGGGCAAAAGGATGCGCCGAAGC
Source
AID BioBrick (BBa_K103001) and self-designed primers