Difference between revisions of "Part:BBa K861036"
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<partinfo>BBa_K861036 short</partinfo>
 
<partinfo>BBa_K861036 short</partinfo>
  
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This part was used to produce protein FadA and FadB. IPTG.......... Together with other Fad proteins that can be induced by IPTG
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To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. This part was used to produce protein FadA and FadB.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:22, 18 September 2012

IPTG induced FadA and FadB

To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. This part was used to produce protein FadA and FadB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 168
    Illegal BglII site found at 1044
    Illegal BglII site found at 1427
    Illegal BglII site found at 1574
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 658
  • 1000
    COMPATIBLE WITH RFC[1000]