Difference between revisions of "Part:BBa K818000:Design"
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===Source=== | ===Source=== | ||
| − | + | Molecular Genetics Group at the University of Groningen, the Netherlands. | |
===References=== | ===References=== | ||
Revision as of 15:01, 14 September 2012
Integration vector for Bacillus subtilis derived from pSac-Cm
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5207
Illegal XbaI site found at 5218
Illegal SpeI site found at 1
Illegal PstI site found at 12 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5207
Illegal SpeI site found at 1
Illegal PstI site found at 12 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5207
Illegal BamHI site found at 180 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5207
Illegal XbaI site found at 5218
Illegal SpeI site found at 1
Illegal PstI site found at 12 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5207
Illegal XbaI site found at 5218
Illegal SpeI site found at 1
Illegal PstI site found at 12
Illegal NgoMIV site found at 4124 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2051
Illegal SapI.rc site found at 3133
Design Notes
This part backbone plasmid design was derived from pSac-Cm Bacillus subtilis backbone plasmid. It has a terminator in its multiple cloning site and red fluorescent protein between the prefix and suffix sequences. It will integrate to B. subtilis chromosome via double recombination from the plasmid and chromosomal sacA sequences.
Source
Molecular Genetics Group at the University of Groningen, the Netherlands.