Difference between revisions of "Part:BBa K301006:Experience"

Revision as of 16:17, 13 September 2012

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Applications of BBa_K301006

User Reviews

[http://2012.igem.org/Team:University_College_London The 2012 iGem UCL team] generated models for BBa_K301006 using TinkerCell software (Chandran et al., 2009). The chart shows a response of RFP under anaerobic conditions and also the accumulation of T7 RNA polymerase (Part: BBa_K301005) under the control of mNark promoter (Part: BBa_K239006) and T7 promoter (Part: BBa_K301003) (in the chart: T7_RNAP1 and T7_RNAP2 respectively). Simulation demonstrated an increase of RFP expression when it was placed under the control of T7 promoter.

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	[http://2012.igem.org/Team:University_College_London The 2012 iGem UCL team] generated models for [https://parts.igem.org/wiki/index.php?title=Part:BBa_K301006 BBa_K301006] using TinkerCell software (Chandran et al., 2009). The chart shows a response of RFP under anaerobic conditions and also the accumulation of T7 RNA polymerase ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K301005 Part: BBa_K301005]) under the control of mNark promoter (Part: BBa_K239006) and T7 promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K301003 Part: BBa_K301003]) (in the chart: T7_RNAP1 and T7_RNAP2 respectively). Simulation demonstrated an increase of RFP expression when it was placed under the control of T7 promoter.		[http://2012.igem.org/Team:University_College_London The 2012 iGem UCL team] generated models for [https://parts.igem.org/wiki/index.php?title=Part:BBa_K301006 BBa_K301006] using TinkerCell software (Chandran et al., 2009). The chart shows a response of RFP under anaerobic conditions and also the accumulation of T7 RNA polymerase ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K301005 Part: BBa_K301005]) under the control of mNark promoter (Part: BBa_K239006) and T7 promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K301003 Part: BBa_K301003]) (in the chart: T7_RNAP1 and T7_RNAP2 respectively). Simulation demonstrated an increase of RFP expression when it was placed under the control of T7 promoter.

−	[[Image:UCL2012-RFPgraph.jpg\|thumb\|center\|700px\|'''Simulation of RFP accumulation over the time under anaerobic conditions''']]	+	[[Image:UCL2012-RFPgraph.jpg\|thumb\|center\|700px\|'''Simulation of RFP accumulation over the time under anaerobic conditions'''\|center\|]]