Difference between revisions of "Part:BBa K784000"

Revision as of 15:19, 13 September 2012

pT7+RBS+Alkaline Phosphatase (pHO)+Terminator

This part consists of T7 RNA polymerase induced promoter followed by an RBS and the ‎Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.‎
The part's purpose is to monitor the translation of the T7 RNA polymerase with the Alkaline ‎Phosphatase gene which can be quantified with the following assay PNP (para-nitrophenol phosphate) hydrolysis assay that has been ‎made by Arkin Lab group in 2006.‎
The Alkaline Phosphatase + RBS part is between XmaI and XhoI restriction sites and ‎therefore can be excised out by these enzymes. ‎

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1512
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1512
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1512
Illegal XhoI site found at 24
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1512
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1512
Illegal NgoMIV site found at 351
Illegal NgoMIV site found at 798
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 This part consists of T7 RNA polymerase induced promoter followed by an RBS and the &#8206;Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.&#8206;<br>
-The part's purpose is to monitor the translation of the T7 RNA polymerase with the Alkaline &#8206;Phosphatase gene which can be quantified with the following assay [[https://parts.igem.org/Arkin_JCA_PNPAssay  [PNP (para-nitrophenol phosphate) hydrolysis assay] that has been &#8206;made by Arkin Lab group in 2006.&#8206;<br>
+The part's purpose is to monitor the translation of the T7 RNA polymerase with the Alkaline &#8206;Phosphatase gene which can be quantified with the following assay [https://parts.igem.org/Arkin_JCA_PNPAssay  PNP (para-nitrophenol phosphate) hydrolysis assay] that has been &#8206;made by Arkin Lab group in 2006.&#8206;<br>
 The Alkaline Phosphatase + RBS  part is between XmaI and XhoI restriction sites and &#8206;therefore can be excised out by these enzymes. &#8206;