Difference between revisions of "Part:BBa K784001"
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<partinfo>BBa_K784001 short</partinfo> | <partinfo>BBa_K784001 short</partinfo> | ||
| − | This part's purpose is to testify to the presence of the T7 RNA Polymerase by the xyIE reporter gene from iGEM2006_Edinburgh. It consists of an RNA T7 promoter, an RBS, a reporter gene- xyIE, and a T7 terminator. The assay for xyIE is very simple, refer to the xyIE basic part for instructions. | + | This part's purpose is to testify to the presence of the T7 RNA Polymerase by the xyIE reporter gene from iGEM2006_Edinburgh. It consists of an RNA T7 promoter, an RBS, a reporter gene- xyIE, and a T7 terminator. The assay for xyIE is very simple, refer to the xyIE basic part for instructions. <br> |
The xyIE+RBS part is between XhoI and XmaI restriction sites, therefore can be excised by these enzymes. | The xyIE+RBS part is between XhoI and XmaI restriction sites, therefore can be excised by these enzymes. | ||
Revision as of 14:27, 13 September 2012
pT7_RBS_xyIE reporter gene
This part's purpose is to testify to the presence of the T7 RNA Polymerase by the xyIE reporter gene from iGEM2006_Edinburgh. It consists of an RNA T7 promoter, an RBS, a reporter gene- xyIE, and a T7 terminator. The assay for xyIE is very simple, refer to the xyIE basic part for instructions.
The xyIE+RBS part is between XhoI and XmaI restriction sites, therefore can be excised by these enzymes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 368
Illegal NgoMIV site found at 540
Illegal AgeI site found at 891 - 1000COMPATIBLE WITH RFC[1000]