Difference between revisions of "Part:BBa K911003:Experience"

Revision as of 16:03, 22 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K911003

This riboswitch came to us from the Yale team in the following construct:

File:OriginalFluorideConstruct.jpg

To verify that this part worked before undertaking more complex assays, we decided to run an assay with X-Gal to give us some qualititative data about its function. The results of this assay are shown below:

The numbers on the tops of the tubes represent the concentration (in mM) of NaF in each culture. The amount of blue is proportional to the amount of β-galactosidase activity present in the culture, which in turn is regulated by the riboswitch. This qualatative assay demonstrated that our part worked.

To get more quantatative data, we performed a Miller assay.

User Reviews

UNIQ14bf8011116ffdd3-partinfo-00000000-QINU UNIQ14bf8011116ffdd3-partinfo-00000001-QINU

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 __NOTOC__
 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K911003===
+This riboswitch came to us from the Yale team in the following construct:
+[[Image:OriginalFluorideConstruct.jpg]]
+To verify that this part worked before undertaking more complex assays, we decided to run an assay with X-Gal to give us some qualititative data about its function. The results of this assay are shown below:
+[[Image:FluorideXgal.jpg]]
+The numbers on the tops of the tubes represent the concentration (in mM) of NaF in each culture. The amount of blue is proportional to the amount of &beta;-galactosidase activity present in the culture, which in turn is regulated by the riboswitch. This qualatative assay demonstrated that our part worked.
+To get more quantatative data, we performed a Miller assay.
 ===User Reviews===