Difference between revisions of "Part:BBa K801999:Design"
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<partinfo>BBa_K801999 short</partinfo>
 
<partinfo>BBa_K801999 short</partinfo>
  
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+
<br>'''Keywords:'''
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'''Keywords:'''
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fluorescent, reporter  
 
fluorescent, reporter  
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'''Abbreviations:'''
+
<br>'''Abbreviations:'''
 
*GFP = Green Fluorescent Protein
 
*GFP = Green Fluorescent Protein
  
  
 
===Design Notes===
 
===Design Notes===
−
'''Other versions of this BioBrick:'''<br>
+
'''Other versions of this BioBrick:'''
−
<!--"The BioBrick Bba_??? encodes the the same functionality but instead..."<br>-->
+
*The BioBrick Bba_??? encodes a yellow fluorescent protein (mVenus)derived from GFP.
 +
*The BioBrick Bba_??? encodes GFP in RFC25 for protein fusions.
  
 
'''Cloning details:'''<br>
 
'''Cloning details:'''<br>
−
<!--"Part was designed in RFC10/RFC23/RFC25"<br>-->
+
*Part was designed in RFC10.
−
<!--"Mutation C889G to delete XbaI restriction site"<br>-->
+
*Mutation C889G to delete XbaI restriction site"<br>-->
 
<!--"Part was truncated upstream/downstream compared to template in order to ???"<br>-->
 
<!--"Part was truncated upstream/downstream compared to template in order to ???"<br>-->
 
<!--"The correctness of part was checked by sequencing" or "... was not yet checked by sequencing"<br>-->
 
<!--"The correctness of part was checked by sequencing" or "... was not yet checked by sequencing"<br>-->

Revision as of 19:26, 11 September 2012

Test page for standardized BioBrick part descriptions


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Keywords:
Abbreviations:

Design Notes

Other versions of this BioBrick:

Cloning details:

Protein coding:

Enzymatic activity:

Cytotoxicity:

Source

Source:

Organism:

References

Literature references:

Seqeuence references:

Structure reference:


TUM12-Test page1.png


Test page for standardized BioBrick part descriptions


Keywords: fluorescent, reporter
Abbreviations:

  • GFP = Green Fluorescent Protein


Design Notes

Other versions of this BioBrick:

  • The BioBrick Bba_??? encodes a yellow fluorescent protein (mVenus)derived from GFP.
  • The BioBrick Bba_??? encodes GFP in RFC25 for protein fusions.

Cloning details:

  • Part was designed in RFC10.
  • Mutation C889G to delete XbaI restriction site"
    -->

Protein coding:

Enzymatic activity:

Cytotoxicity:

Source

Source: Synthesized

Organism:

References

Literature references:

  • [http://www.ncbi.nlm.nih.gov/pubmed/20010584 Pubmed: Prasher, 1995: Using GFP to see the light.(Review)]
  • [http://www.ncbi.nlm.nih.gov/pubmed/20010584 Pubmed: Prasher, 1995: Using GFP to see the light.(Review)]

Seqeuence references:

  • [http://www.ncbi.nlm.nih.gov/nuccore/X83959.1: A.victoria mRNA for green fluorescent protein (ID:gfp1)]
  • [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR011584 Interpro: Green fluorescent protein-related ]
  • [http://www.uniprot.org/uniprot/P42212 Uniprot: Green fluorescent protein]
  • [http://pfam.sanger.ac.uk/family/PF01353 Pfam: Green fluorescent protein]

Structure reference:

  • [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EMA PDB: Green Fluorescent Protein from Aequorea victoria]