Difference between revisions of "Part:BBa K861061"

Revision as of 05:26, 11 September 2012

Efficacy testing RFP generator of BBa_K861060 (PfadR)

We placed BBa_I13507 downstream of BBa_K861060 (PfadR) in plasmid pSB6A1. M9 medium with oleic acid as sole carbon source to test was used to test the efficacy of the promoter. Specifically, oleic acid was emulsified with 10% Triton X100 with volume 1:1. Then various volume of mixture is added to M9 medium with 0.2% triton X100. We vortex E.coli Dh5 alpha in the medium for 24h and using SpectraMax M2 plate reader to see its OD600 and fluorescence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 657
Illegal AgeI site found at 769
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K861061 short</partinfo>
-We use BBa_I13507 test function of BBa_K861060 (PfadR) in M9 medium with oleic acid as sole carbon source in plasmid pSB6A1. Specifically, oleic acid was emulsified with 10% Triton X100 with volume 1:1. Then various volume of mixture is added to M9 medium with 0.2% triton X100. We vortex E.coli Dh5 alpha in the medium for 24h and using SpectraMax M2 plate reader to see its OD600 and fluorescence.
+We placed BBa_I13507 downstream of BBa_K861060 (PfadR) in plasmid pSB6A1. M9 medium with oleic acid as sole carbon source to test was used to test the efficacy of the promoter. Specifically, oleic acid was emulsified with 10% Triton X100 with volume 1:1. Then various volume of mixture is added to M9 medium with 0.2% triton X100. We vortex E.coli Dh5 alpha in the medium for 24h and using SpectraMax M2 plate reader to see its OD600 and fluorescence.
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