Difference between revisions of "Part:BBa K823019"
Line 4: | Line 4: | ||
This lacZ gene is derived from the Bacillus reporter vector pAC6. It is constructed in the [[Help:Assembly_standard_25|Freiburg standard]] with RBS. | This lacZ gene is derived from the Bacillus reporter vector pAC6. It is constructed in the [[Help:Assembly_standard_25|Freiburg standard]] with RBS. | ||
− | + | lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (''ortho''-Nitrophenyl-β-galactoside). | |
prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC | prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC | ||
suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT | suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT | ||
− | |||
− | |||
Revision as of 19:12, 23 September 2012
LacZ (RBS+Freiburg standard)
This lacZ gene is derived from the Bacillus reporter vector pAC6. It is constructed in the Freiburg standard with RBS.
lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (ortho-Nitrophenyl-β-galactoside).
prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC
suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
We used this lacZ gene to evaluate our promoters.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]