Difference between revisions of "Part:BBa K823036"
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cMyc-tag with RBS in Freiburg standard. | cMyc-tag with RBS in Freiburg standard. | ||
+ | |||
+ | |||
prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC | prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC | ||
+ | |||
suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT | suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT | ||
− | This is a part created by the LMU-Munich 2012 team. See here for other usefull parts of the [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''BacillusB'''''io'''B'''rick'''B'''ox]. | + | |
+ | This is a part created by the LMU-Munich 2012 team. We added five tags to the registry, all in the Freiburg standard for N-and C-terminal fusions: | ||
+ | |||
+ | [Part:BBa_K823034|3x Flag tag],[Part:BBa_K823035|HA-tag],[Part:BBa_K823036|cMyc-tag],[Part:BBa_K823037|3x Flag tag],[Part:BBa_K823038|3x Flag tag] | ||
+ | |||
+ | |||
+ | See here for other usefull parts of the [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''BacillusB'''''io'''B'''rick'''B'''ox]. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 09:58, 8 September 2012
cMyc-tag (Freiburg standard+RBS)
cMyc-tag with RBS in Freiburg standard.
prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC
suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
This is a part created by the LMU-Munich 2012 team. We added five tags to the registry, all in the Freiburg standard for N-and C-terminal fusions:
[Part:BBa_K823034|3x Flag tag],[Part:BBa_K823035|HA-tag],[Part:BBa_K823036|cMyc-tag],[Part:BBa_K823037|3x Flag tag],[Part:BBa_K823038|3x Flag tag]
See here for other usefull parts of the [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks BacillusBioBrickBox].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 24
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]