Difference between revisions of "Part:BBa K823034"

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<partinfo>BBa_K823034 short</partinfo>
 
<partinfo>BBa_K823034 short</partinfo>
  
3x Flag tag with RBS in Freiburg standard.
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3x Flag tag with RBS in [[Help:Assembly_standard_25|Freiburg standard]].
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prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC
 
prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC
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suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
 
suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
  
<!-- Add more about the biology of this part here
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This is a part created by the LMU-Munich 2012 team.  We added five tags to the registry, all in the Freiburg standard for N-and C-terminal fusions:
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[[Part:BBa_K823034|3x Flag tag]]
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[[Part:BBa_K823035|HA-tag]]
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[[Part:BBa_K823036|cMyc-tag]]
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[[Part:BBa_K823037|His-tag]]
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[[Part:BBa_K823038|Streptavidin-tag]]
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Visit our project page for more usefull parts of our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''BacillusB'''''io'''B'''rick'''B'''ox].
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===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 11:49, 8 September 2012

3x FLAG tag (Freiburg standard+RBS)

3x Flag tag with RBS in Freiburg standard.


prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC

suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT


This is a part created by the LMU-Munich 2012 team. We added five tags to the registry, all in the Freiburg standard for N-and C-terminal fusions:

3x Flag tag

HA-tag

cMyc-tag

His-tag

Streptavidin-tag


Visit our project page for more usefull parts of our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks BacillusBioBrickBox].



Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]