Difference between revisions of "Part:BBa K763001:Experience"

 
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<partinfo>BBa_K763001 1</partinfo>
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<I>Shadowburn</I>
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Our team 2019 GDSYZX run the enzyme digestion verification and sequence test of plasmid GroE-AsRed2, then test the fluorescence intensity under different heat-induce time. (set gradient between 0 and 30 minutes.)</br>
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We place the shaken fugus solution into 18℃,28℃,37℃, 42℃, and 55℃ pots for 10 minutes and cultivate the solution in static under room temperature. We test the OD600 value and Florence intensity 0 min, 30 min, 60 min, 90min, 120min,150min, 180min after the heat shock transformation.
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(measure condition: wavelength of excited light: 576nm, wavelength of emitting light: 592nm, E.coli competent :DH5α, chloramphenicol LB culture medium , Endonuclease XbaI and SpeI: enzyme test of GroE-AsRed2 plasmid.)</br>
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The result indicates that the fluorescence intensity is the highest under 55 ℃ and that the fluorescence intensity all tends to reach their maximum at about 125 minutes after heat shock transformation under different temperature.
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Revision as of 03:57, 13 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K763001

User Reviews

UNIQ7f9424aa5b1ff8a3-partinfo-00000000-QINU

BBa_K763001 1 Not understood Shadowburn

Our team 2019 GDSYZX run the enzyme digestion verification and sequence test of plasmid GroE-AsRed2, then test the fluorescence intensity under different heat-induce time. (set gradient between 0 and 30 minutes.)</br> We place the shaken fugus solution into 18℃,28℃,37℃, 42℃, and 55℃ pots for 10 minutes and cultivate the solution in static under room temperature. We test the OD600 value and Florence intensity 0 min, 30 min, 60 min, 90min, 120min,150min, 180min after the heat shock transformation. (measure condition: wavelength of excited light: 576nm, wavelength of emitting light: 592nm, E.coli competent :DH5α, chloramphenicol LB culture medium , Endonuclease XbaI and SpeI: enzyme test of GroE-AsRed2 plasmid.)</br> The result indicates that the fluorescence intensity is the highest under 55 ℃ and that the fluorescence intensity all tends to reach their maximum at about 125 minutes after heat shock transformation under different temperature.

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UNIQ7f9424aa5b1ff8a3-partinfo-00000002-QINU