Difference between revisions of "Part:BBa K775003"
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| − | BBa_K775003 encodes a chimeric diacetyl-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the C. elegans 7-transmembrane receptor ODR-10, flanked by the N- and C- terminals of the rat OR RI7. | + | BBa_K775003 encodes a chimeric diacetyl-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the C. elegans 7-transmembrane receptor ODR-10, flanked by the N- and C- terminals of the rat OR RI7. This part is an improvement of [https://parts.igem.org/Part:BBa_K211002 BBa_K211002] made by 2009 Hongkong university. A GPD promoter, Kozak sequence, and CYC1 terminator have been added. |
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Latest revision as of 12:21, 26 September 2012
RI7-odr10 (diacetyl) chimeric GPCR with promoter and terminator
BBa_K775003 encodes a chimeric diacetyl-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the C. elegans 7-transmembrane receptor ODR-10, flanked by the N- and C- terminals of the rat OR RI7. This part is an improvement of BBa_K211002 made by 2009 Hongkong university. A GPD promoter, Kozak sequence, and CYC1 terminator have been added.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 113
Illegal XhoI site found at 885 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]