Difference between revisions of "Part:BBa K808032"

Revision as of 14:00, 23 September 2012

Arabinose regulated RBS-PhoA-His6tag-pNBEst13-Myctag-EstA

This is a composite of 2 different BioBricks formed by a standard assembly, forming a scar in between. It contains the following units: AraC-AraO2-AraO1-AraPromo(PBAD-RBS-PhoA-His6tag-pNBEst13-Myctag-EstA which is similiar to BBa_K808000 & BBa_K808030 It is an operon, being induced by adding 0.02% to 0.5% of L-Arabinose. It is made for regulating the cell surface expression of our PET cleaving enzyme pNB-Est13, which is anchored C-terminal to EstA.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2392
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2918
Illegal NgoMIV site found at 3056
Illegal NgoMIV site found at 3596
Illegal NgoMIV site found at 3965
Illegal AgeI site found at 979
Illegal AgeI site found at 2463
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K808032 short</partinfo>
-This a a composite of 2 different BioBricks by an standard assembly, forming a scar in between.
+This is a composite of 2 different BioBricks formed by a standard assembly, forming a scar in between.
 It contains the following units:
-AraC-AraO2-AraO1-AraPromo(PBAD)- RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
+AraC-AraO2-AraO1-AraPromo(PBAD-RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
 which is similiar to BBa_K808000 & BBa_K808030
-Its a regulatory system, being induced by adding 1 to 2% of mw% L-Arabinose. It is made for regulating the cell surface Expression of our PET cleaving enzyme pNB-Est13, which is anchored c-terminal to EstA.
+It is an operon, being induced by adding 0.02% to 0.5% of L-Arabinose. It is made for regulating the cell surface expression of our PET cleaving enzyme pNB-Est13, which is anchored C-terminal to EstA.