Difference between revisions of "Part:BBa K808030:Design"
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===References=== | ===References=== | ||
+ | * Fakult, M., & Enzymtechnologie, M. (2006). biochemische Charakterisierung Dialkylphthalat spaltender Esterasen. | ||
+ | * Becker, S., Theile, S., Heppeler, N., Michalczyk, A., Wentzel, A., Wilhelm, S., Jaeger, K.-E., et al. (2005). A generic system for the Escherichia coli cell-surface display of lipolytic enzymes. FEBS letters, 579(5), 1177–82. doi:10.1016/j.febslet.2004.12.087 | ||
+ | * Yang, T. H., Pan, J. G., Seo, Y. S., & Rhee, J. S. (2004). Use of Pseudomonas putida EstA as an Anchoring Motif for Display of a Periplasmic Enzyme on the Surface of Escherichia coli, 70(12), 6968–6976. doi:10.1128/AEM.70.12.6968 |
Latest revision as of 21:18, 24 September 2012
RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1174
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1700
Illegal NgoMIV site found at 1838
Illegal NgoMIV site found at 2378
Illegal NgoMIV site found at 2747
Illegal AgeI site found at 1245 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We got it synthesized by GENEART but added the RBS via PCR.
Source
This is no natural or even native construct. We got it synthesized by GENEART. but added the RBS via PCR.
References
- Fakult, M., & Enzymtechnologie, M. (2006). biochemische Charakterisierung Dialkylphthalat spaltender Esterasen.
- Becker, S., Theile, S., Heppeler, N., Michalczyk, A., Wentzel, A., Wilhelm, S., Jaeger, K.-E., et al. (2005). A generic system for the Escherichia coli cell-surface display of lipolytic enzymes. FEBS letters, 579(5), 1177–82. doi:10.1016/j.febslet.2004.12.087
- Yang, T. H., Pan, J. G., Seo, Y. S., & Rhee, J. S. (2004). Use of Pseudomonas putida EstA as an Anchoring Motif for Display of a Periplasmic Enzyme on the Surface of Escherichia coli, 70(12), 6968–6976. doi:10.1128/AEM.70.12.6968