Difference between revisions of "Part:BBa K754000:Design"
Jesterface (Talk | contribs) (→Design Notes) |
Jesterface (Talk | contribs) (→Design Notes) |
||
| Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | Certain punctual changes on the promoter sequence had to be done in order tu ensure that the biobrick was compatible with all the registry standarized enzymes. Specifically the third nucelotide of the sequence had to be changed by an adenine in order to remove an XbaI target | + | Certain punctual changes on the promoter sequence had to be done in order tu ensure that the biobrick was compatible with all the registry standarized enzymes. Specifically the third nucelotide of the sequence (initially a guanine) had to be changed by an adenine in order to remove an XbaI target |
===Source=== | ===Source=== | ||
Latest revision as of 18:21, 29 August 2012
S. elongatus PCC7942 psbAI promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Certain punctual changes on the promoter sequence had to be done in order tu ensure that the biobrick was compatible with all the registry standarized enzymes. Specifically the third nucelotide of the sequence (initially a guanine) had to be changed by an adenine in order to remove an XbaI target
Source
The sequence of this part comes from the AM 2195 plasmid developed by doctor Susan Golden.