Difference between revisions of "Part:BBa K731710"
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The vector was obained by mutagenesis of [[BBa_K731700]], replacing T7 promoter with pTAC. It contains Ampicillin resistance gene and is inducible by IPTG (lacO).
 
The vector was obained by mutagenesis of [[BBa_K731700]], replacing T7 promoter with pTAC. It contains Ampicillin resistance gene and is inducible by IPTG (lacO).
 
The combined use of BBa_K731710 and [[https://parts.igem.org/Part:BBa_K731700:Experience#Applications_of_BBa_K731700]] allow also to analyse any potential difference in terminators' activity due to different polymerases.
 
The combined use of BBa_K731710 and [[https://parts.igem.org/Part:BBa_K731700:Experience#Applications_of_BBa_K731700]] allow also to analyse any potential difference in terminators' activity due to different polymerases.
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[[b0010]]
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The two fluorescent proteins have partially overlapping emission spectra, thus, to avoid unwanted interferences, the fluorimetric measures should be taken with these parameters:
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Venus excitation: 485nm, Venus emission: 528nm
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Cherry excitation: 528nm, Cherry emission: 615nm.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:56, 24 August 2012

Platform for terminators analysis under the control of tac promoter

This part is used to analyse terminators: two subsequent fluorescent proteins (mCherry and A206K Venus)allow to obtain several information about terminator's presence effect. The vector was obained by mutagenesis of BBa_K731700, replacing T7 promoter with pTAC. It contains Ampicillin resistance gene and is inducible by IPTG (lacO). The combined use of BBa_K731710 and [[1]] allow also to analyse any potential difference in terminators' activity due to different polymerases. b0010

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6865
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 6871
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6865
    Illegal BglII site found at 6011
    Illegal BamHI site found at 6847
    Illegal XhoI site found at 753
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 6865
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 6865
    Plasmid lacks a suffix.
    Illegal XbaI site found at 6880
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 714
    Illegal NgoMIV site found at 1051
    Illegal NgoMIV site found at 4231
    Illegal NgoMIV site found at 4391
    Illegal NgoMIV site found at 5979
    Illegal AgeI site found at 6815
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2228
    Illegal SapI.rc site found at 3310