Difference between revisions of "Part:BBa K731500"

 
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<partinfo>BBa_K731500 short</partinfo>
 
<partinfo>BBa_K731500 short</partinfo>
  
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This is E.coli lactose-inducibile promoter.
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This composite part consist of K731300 part (lacI-lacIq) and a tac promoter followed
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by a lac operator in the forward direction. It is a composite part ready to use
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lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription.
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and facilitate the expression of a gene of interest under the control of IPTG
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Low, constitutive levels of transcription can be seen anyhow.
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and a strong E.coli promoter.
  
 
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Revision as of 08:04, 29 August 2012

[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward

This composite part consist of K731300 part (lacI-lacIq) and a tac promoter followed by a lac operator in the forward direction. It is a composite part ready to use and facilitate the expression of a gene of interest under the control of IPTG and a strong E.coli promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]