Difference between revisions of "Part:BBa K823025:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K823025 short</partinfo> | <partinfo>BBa_K823025 short</partinfo> | ||
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===Source=== | ===Source=== | ||
| − | This vector is an igem compatible version of the pAH328 Bacillus reporter | + | This vector is an igem compatible version of the pAH328 ''Bacillus subtilis'' reporter vector. |
===References=== | ===References=== | ||
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| + | [http://www.ncbi.nlm.nih.gov/pubmed/20709900 Schmalisch ''et al''.] | ||
Latest revision as of 08:46, 26 September 2012
pSBBs3C-luxABCDE (lux reporter vector for B.subtilis)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 10619
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 10625 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 10619
Illegal BglII site found at 5170
Illegal BamHI site found at 5705 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 10619
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 10619
Plasmid lacks a suffix.
Illegal XbaI site found at 10634
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 9536
Illegal AgeI site found at 576 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 7576
Illegal BsaI.rc site found at 3489
Illegal SapI site found at 1095
Illegal SapI.rc site found at 8658
Design Notes
We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.
Source
This vector is an igem compatible version of the pAH328 Bacillus subtilis reporter vector.
References
[http://www.ncbi.nlm.nih.gov/pubmed/20709900 Schmalisch et al.]