Difference between revisions of "Part:BBa K731020:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | CysE was inserted after araC-pBAD in his vector, cutting the former with | + | CysE was inserted after araC-pBAD in his vector, cutting the former with S&P and the latter with X&P. |
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===Source=== | ===Source=== | ||
Revision as of 08:03, 21 August 2012
Inducible araC-pBAD promoter regulating WT CysE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1909 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
CysE was inserted after araC-pBAD in his vector, cutting the former with S&P and the latter with X&P.
Source
K731200, K731000