Difference between revisions of "Part:BBa K887000"
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<partinfo>BBa_K887000 short</partinfo> | <partinfo>BBa_K887000 short</partinfo> | ||
+ | In traditional genetic engineering method, we use strong promoter to initiate our genes, but this way E.coli will overexpress the proteins we need in synthetic pathway. However, this overexpression of target proteins will cause E.coli wastes its limited growth resources, or the activity and performance of the enzymes may be too low. In this situation, it unbalances the synthetic pathway, and the production of isobutanol will not be optimum. This is also a problem in the production of isobutanol which is poisonous to E.coli. | ||
Revision as of 02:55, 20 August 2012
Plac+B0034+zif268+alsS+B0034+PBSII+ilvC+B0034+HIVC+ilvD+37℃ induced RBS+tetR+double terminator
In traditional genetic engineering method, we use strong promoter to initiate our genes, but this way E.coli will overexpress the proteins we need in synthetic pathway. However, this overexpression of target proteins will cause E.coli wastes its limited growth resources, or the activity and performance of the enzymes may be too low. In this situation, it unbalances the synthetic pathway, and the production of isobutanol will not be optimum. This is also a problem in the production of isobutanol which is poisonous to E.coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 6049
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 6938
Illegal AgeI site found at 3158
Illegal AgeI site found at 4143 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2744
Illegal BsaI site found at 5835
Illegal BsaI site found at 6092
Illegal BsaI.rc site found at 836
Illegal BsaI.rc site found at 1430
Illegal BsaI.rc site found at 3563