Difference between revisions of "Part:BBa K917003:Experience"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 5: | Line 4: | ||
===Applications of BBa_K917003=== | ===Applications of BBa_K917003=== | ||
| + | We have tested the expression of NapC protein under control of lac promoter in ''ccm'' transformed cells (''ccm'' gene cluster BioBrick was transferred into pSB4K5 vector to allow for double epxression of ''ccm'' and ''napC'' gene). Cell pellets were scanned prior to sonication and ''napC'' transformed cells show slightly more intense colour, indicating higher concentration of haem in cells. | ||
| + | [[Image:Cytochrome compare.jpg]] | ||
| + | Haem staining results suggest NapC expression in E coli transformed with ''plac-napC'' and ''plac-ccm''. The additional band indicated by an arrow in lane 7 represents NapC protein. | ||
| + | [[Image:Gel_haem_compare_.jpg]] | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K917003 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K917003 StartReviews</partinfo> | ||
Latest revision as of 11:41, 30 October 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K917003
We have tested the expression of NapC protein under control of lac promoter in ccm transformed cells (ccm gene cluster BioBrick was transferred into pSB4K5 vector to allow for double epxression of ccm and napC gene). Cell pellets were scanned prior to sonication and napC transformed cells show slightly more intense colour, indicating higher concentration of haem in cells.
Haem staining results suggest NapC expression in E coli transformed with plac-napC and plac-ccm. The additional band indicated by an arrow in lane 7 represents NapC protein.
User Reviews
UNIQce2b637bf1c0918a-partinfo-00000000-QINU UNIQce2b637bf1c0918a-partinfo-00000001-QINU