Difference between revisions of "Part:BBa J176132:Design"

(→‎Design Notes)
(→‎Source)
 
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===Source===
 
===Source===
  
−
TBA
+
* Synthetic.
  
 
===References===
 
===References===

Latest revision as of 18:59, 1 August 2012

PLrigid4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
    Illegal PstI site found at 95
    Illegal PstI site found at 110
    Illegal PstI site found at 230
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
    Illegal PstI site found at 95
    Illegal PstI site found at 110
    Illegal PstI site found at 230
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
    Illegal PstI site found at 95
    Illegal PstI site found at 110
    Illegal PstI site found at 230
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
    Illegal PstI site found at 95
    Illegal PstI site found at 110
    Illegal PstI site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Synthesized by GenScript; cloned into the pUC57 vector.
  • The PLrigid4 DNA sequence is not simply four tandem copies of the PLrigid DNA sequence. The sequence was edited manually to reduce DNA repetitiveness.

Source

  • Synthetic.

References