Difference between revisions of "Part:BBa K741000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We construct this part by PCR with design primers which can reversely arrange the sites for EcoRI, XbaI, SpeI, PstI. The template DNA we use is the genome of lambda phage. | |
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| + | You need a promoter and a terminator but no RBS when using this part. | ||
===Source=== | ===Source=== | ||
Revision as of 19:00, 2 October 2012
anticro
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 134
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We construct this part by PCR with design primers which can reversely arrange the sites for EcoRI, XbaI, SpeI, PstI. The template DNA we use is the genome of lambda phage.
You need a promoter and a terminator but no RBS when using this part.
Source
BBa_I759002