Difference between revisions of "Part:BBa K823003:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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This part was used as a part of the Bee subtilis, iGEM METU 2013 project. We did alignment by using CLUSTAL W between two Pveg promoters; K143012 and K823003. The result is as follows:
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http://wiki.metuigem.com/images/1375868_10202188366932242_1731194941_n.jpg
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K823003 sequence has 237 bp and K143012 sequence has 97 bp as seen in the figure. We can conclude that K823003 includes K143012. After BLASTing the remaining part of the K823003 which is not same as K143012 there were no results for specific sequences for polimerase binding. This shows K823003 has alike promoter strength as K143012. Only the primers were desinged further from the main promoter region. So for this promoter we can use the promoter strength of the K143012.
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Revision as of 02:46, 5 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K823003

User Reviews

UNIQ5b3035b18132143c-partinfo-00000000-QINU

•••••

This part was used as a part of the Bee subtilis, iGEM METU 2013 project. We did alignment by using CLUSTAL W between two Pveg promoters; K143012 and K823003. The result is as follows:

http://wiki.metuigem.com/images/1375868_10202188366932242_1731194941_n.jpg

K823003 sequence has 237 bp and K143012 sequence has 97 bp as seen in the figure. We can conclude that K823003 includes K143012. After BLASTing the remaining part of the K823003 which is not same as K143012 there were no results for specific sequences for polimerase binding. This shows K823003 has alike promoter strength as K143012. Only the primers were desinged further from the main promoter region. So for this promoter we can use the promoter strength of the K143012.

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UNIQ5b3035b18132143c-partinfo-00000002-QINU