Difference between revisions of "Part:BBa K912000"

Revision as of 23:11, 3 October 2012

Citrobacter braakii phytase

Phytase is an enzyme that breaks down phytic acid. This particular phytase was cloned from Citrobacter braakii, a gram-negative bacteria. This phytase acts to break down phytic acid into inositol, releasing phosphate groups on the phytate. If the phytate has chelated any other particle, phytase should break down the phytate to the point where the chelated particle is released.

Usage and Biology

Once expressed, the E. coli cell had to be lysed in order to release the phytase into the extra-cellular environment. We used Part:BBa_K112808 in order to lyse the cell. Once the phytase is released it is free to act upon the phytic acid.

Please visit our [http://2012.igem.org/Team:Northwestern wiki] for more information!

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1005
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 67

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K912000 short</partinfo>
-Part to produce C. braakii phytase. This phytase acts to break down phytic acid into inositol, releasing chelated iron and other micronutrients.
+Phytase is an enzyme that breaks down phytic acid. This particular phytase was cloned from Citrobacter braakii, a gram-negative bacteria. This phytase acts to break down phytic acid into inositol, releasing phosphate groups on the phytate. If the phytate has chelated any other particle, phytase should break down the phytate to the point where the chelated particle is released.
+==Usage and Biology==
+Once expressed, the E. coli cell had to be lysed in order to release the phytase into the extra-cellular environment. We used [[Part:BBa_K112808]] in order to lyse the cell. Once the phytase is released it is free to act upon the phytic acid.
-<!-- Add more about the biology of this part here
+'''Please visit our [http://2012.igem.org/Team:Northwestern wiki] for more information!'''
-===Usage and Biology===
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