Difference between revisions of "Part:BBa K917000:Experience"

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Expression of this gene allows growth of E. coli K12-derived strains with sucrose as sole carbon source. We (Edinburgh 2012) have demonstrated this using the part cloned in pSB1C3, with no added promoter; this allowed good growth on M9 agar plates with sucrose added as sole carbon source, whereas vector control cells failed to grow.
 
Expression of this gene allows growth of E. coli K12-derived strains with sucrose as sole carbon source. We (Edinburgh 2012) have demonstrated this using the part cloned in pSB1C3, with no added promoter; this allowed good growth on M9 agar plates with sucrose added as sole carbon source, whereas vector control cells failed to grow.
  
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[[Image:CONsample.jpg]] Control plate with no sugars
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<gallery widths=250px heights=250px>
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Image:CONsample.jpg|Control plate with no sugars
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Image:SUCsample.jpg|Plate with sucrose as carbon source
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Image:GLUsample.jpg|Plate with glucose as carbon source
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</gallery>
  
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[[Image:SUCsample.jpg]] Plate with sucrose as carbon source
 
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[[Image:GLUsample.jpg]] Plate with glucose as carbon source
 
  
 
===User Reviews===
 
===User Reviews===

Revision as of 19:37, 19 June 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K917000

Expression of this gene allows growth of E. coli K12-derived strains with sucrose as sole carbon source. We (Edinburgh 2012) have demonstrated this using the part cloned in pSB1C3, with no added promoter; this allowed good growth on M9 agar plates with sucrose added as sole carbon source, whereas vector control cells failed to grow.

  • Control plate with no sugars

  • Plate with sucrose as carbon source

  • Plate with glucose as carbon source


User Reviews

UNIQcf90464bc49ad888-partinfo-00000001-QINU UNIQcf90464bc49ad888-partinfo-00000002-QINU