Difference between revisions of "Part:BBa K611066"

Latest revision as of 19:23, 15 May 2012

cI Lambda Coding region without Barcode testing construct

This part is designed for testing the promoter region in the barcode region of cI Lambda (BBa_C0051). The barcode has been removed, but the rest of the BBa_C0051 biobrick has been unchanged. It has been placed upstream of an RBS (BBa_B0034) and RFP (BBa_E1010) in the BBa_J61002 plasmid.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2927
Illegal XbaI site found at 2942
Illegal SpeI site found at 2
Illegal PstI site found at 885
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2927
Illegal SpeI site found at 2
Illegal PstI site found at 885
Illegal NotI site found at 878
Illegal NotI site found at 2933
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2927
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2927
Illegal XbaI site found at 2942
Illegal SpeI site found at 2
Illegal PstI site found at 885
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2927
Illegal XbaI site found at 2942
Illegal SpeI site found at 2
Illegal PstI site found at 885
Illegal AgeI site found at 581
Illegal AgeI site found at 693
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1966

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K611066 short</partinfo>
-This part is designed for testing the promoter region in the barcode region of cI Lambda (BBa_C0051).  The barcode has been removed, but the rest of the BBa_C0051 biobrick has been unchanged.
+This part is designed for testing the promoter region in the barcode region of cI Lambda (BBa_C0051).  The barcode has been removed, but the rest of the BBa_C0051 biobrick has been unchanged.  It has been placed upstream of an RBS (BBa_B0034) and RFP (BBa_E1010) in the BBa_J61002 plasmid.
 <!-- Add more about the biology of this part here