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Revision as of 19:01, 2 May 2012
The 3A Assembly Kit | |
You've just received the 3A Assembly Kit! We hope your team will enjoy using the kit and in the process learn about synthetic biology based on standard parts and 3A assembly. Before you start please make sure to...
If you're interested in using the 3A Assembly Kit, and would like to order one, please contact hq (at) igem (dot) org. |
Introduction
The 3A Assembly Kit and following protocols will take you through the process of 3A Assembly, and by the end you will have assembled your own composite part in the lab. The kit includes two parts: Part A (BBa_J04500) and Part B (BBa_J04650), which when assembled together will form a RFP (red fluorescent protein) generator. Your cells will turn red!
Note: The 3A Assembly Kit also includes prepared plasmid DNA for Part A and Part B, so you can skip directly to Step #3: Restriction Digest if you'd like.
Protocols
Step #1: Growing | |
Grow up the E. coli that contains your parts! |
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Step #2: Miniprep | |
Miniprepping cell cultures to extract the DNA for your parts. |
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Step #3: Restriction Digest | |
Cutting the DNA for your parts and the pSB1C3 linearized plasmid backbone in a restriction digest |
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Step #4: Ligation | |
Ligating your two parts together into the pSB1C3 linearized backbone |
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Step #5: Transformation | |
Transform the ligation into E. coli to get your newly assembled composite part. |
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Step #6: Results | |
Post your results! Hopefully you've learned quite a bit while using the 3A Assembly Kit. |