Difference between revisions of "Part:BBa K896937:Design"
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===References=== | ===References=== | ||
Revision as of 17:46, 26 April 2012
colicin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers used in the PCR:
colicin_FP: gcttctag atgagcggtggcgatg(TM:56C) colicin_RP: gct ctgcagcggccgctactagta ttacttaccccgatgaatatcaatatgt (TM:56C)
Digested the PCR product with XbaI and PstI, and ligated the digestion product with a gel purified XbaI,PstI digested pSB1A2 plasmid backbone.
Source
pIGMS5, a new plasmid from clinical Escherichia coli strain