Difference between revisions of "Part:BBa J153001:Design"

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===References===
 
===References===
 +
Brosius, J., Erfle, M. and Storella, J. (1985) Spacing of the -10 and -35 Regions in the Tac Promoter - Effect on Its Invivo Activity. Journal of Biological Chemistry, 260, 3539-3541.
 +
 +
Huang, H.H., Camsund, D., Lindblad, P. and Heidorn, T. (2010) Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology. Nucleic Acids Research, 38, 2577-2593.

Latest revision as of 11:58, 3 February 2012

Ptrc1O


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Ptrc1O, or the trc promoter, was extracted from the pTrc99A plasmid using PCR (Huang etal 2010, Brosius etal 1985).

References

Brosius, J., Erfle, M. and Storella, J. (1985) Spacing of the -10 and -35 Regions in the Tac Promoter - Effect on Its Invivo Activity. Journal of Biological Chemistry, 260, 3539-3541.

Huang, H.H., Camsund, D., Lindblad, P. and Heidorn, T. (2010) Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology. Nucleic Acids Research, 38, 2577-2593.