Difference between revisions of "Part:BBa J153001"
(New page: == ''Ptrc1O'' == P''trc1O'', or the ''trc'' promoter, is a hybrid of the ''Escherichia coli trp'' promoter and the ''lacUV5'' promoter (Brosius etal 1985) [http://www.ncbi.nlm.nih.gov/pu...) |
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'''Usage''' | '''Usage''' | ||
| − | It was characterized with a GFP reporter construct ([[Part:BBa_I13504]]) carried on the broad-host-range shuttle vector pPMQAK1 ([[Part:BBa_J153000]]) to be stronger than both the ''lacZYA'' operon promoter ([[Part:BBa_R0010]]) (ca 35% stronger) and a cI-based TetR-repressible promoter ([[Part:BBa_R0040]]) (ca 320 % stronger) in ''E. coli'' DH5alpha. Characterization in the cyanobacterium ''Synechocystis'' sp. PCC 6803 revealed strong fluorescence from the P''trc1O''-driven construct but no detectable signal from the | + | It was characterized with a GFP reporter construct ([[Part:BBa_I13504]]) carried on the broad-host-range shuttle vector pPMQAK1 ([[Part:BBa_J153000]]) to be stronger than both the ''lacZYA'' operon promoter ([[Part:BBa_R0010]]) (ca 35% stronger) and a cI-based TetR-repressible promoter ([[Part:BBa_R0040]]) (ca 320 % stronger) in ''E. coli'' DH5alpha. Characterization in the cyanobacterium ''Synechocystis'' sp. PCC 6803 revealed strong fluorescence from the P''trc1O''-driven construct but no detectable signal from the “Plac” construct and only a weak signal from the “PtetR” construct. Repression of P''trc1O'' by LacI in ''Synechocystis'' was very modest (Huang etal 2010) [http://www.ncbi.nlm.nih.gov/pubmed/20236988]. |
The P''trc2O'' promoter (Part:BBa_J153002) is of similar strength as P''trc1O'' but significantly more repressed by LacI in ''Synechocystis''. However, there are problems inducing this promoter to its full strength, limiting its usability. | The P''trc2O'' promoter (Part:BBa_J153002) is of similar strength as P''trc1O'' but significantly more repressed by LacI in ''Synechocystis''. However, there are problems inducing this promoter to its full strength, limiting its usability. | ||
Revision as of 11:55, 3 February 2012
Ptrc1O
Ptrc1O, or the trc promoter, is a hybrid of the Escherichia coli trp promoter and the lacUV5 promoter (Brosius etal 1985) [http://www.ncbi.nlm.nih.gov/pubmed/2579077].
It contains the lac O1 operator (proximal to the core promoter) that allows for LacI repression and subsequent induction by IPTG.
Usage
It was characterized with a GFP reporter construct (Part:BBa_I13504) carried on the broad-host-range shuttle vector pPMQAK1 (Part:BBa_J153000) to be stronger than both the lacZYA operon promoter (Part:BBa_R0010) (ca 35% stronger) and a cI-based TetR-repressible promoter (Part:BBa_R0040) (ca 320 % stronger) in E. coli DH5alpha. Characterization in the cyanobacterium Synechocystis sp. PCC 6803 revealed strong fluorescence from the Ptrc1O-driven construct but no detectable signal from the “Plac” construct and only a weak signal from the “PtetR” construct. Repression of Ptrc1O by LacI in Synechocystis was very modest (Huang etal 2010) [http://www.ncbi.nlm.nih.gov/pubmed/20236988].
The Ptrc2O promoter (Part:BBa_J153002) is of similar strength as Ptrc1O but significantly more repressed by LacI in Synechocystis. However, there are problems inducing this promoter to its full strength, limiting its usability.