Difference between revisions of "Part:BBa J176008"

Revision as of 02:52, 11 December 2011

NLS

SV40 nuclear localization sequence

Usage and Biology

Include within the ORF of a protein that you need transport into the nucleus (e.g., a synthetic transcription factor)
FP-NLS fusions are handy for making "glowing" nuclei

PKKKRKV motif from the Simian Virus 40 Large T-antigen. When added to a eukaryotic protein, that protein localizes to the nucleus. in our hands, the localization appears to be very efficient (data coming soon). This tag is very handy for live cell fluorescent labeling, since image processing of glowing nuclei with nice defined edges is much simpler than quantifying diffuse signal in cell bodies with irregular edges.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-* Include within the ORF of a protein that you need to target to the nucleus (e.g., a synthetic transcription factor)
+* Include within the ORF of a protein that you need transport into the nucleus (e.g., a synthetic transcription factor)
 * FP-NLS fusions are handy for making "glowing" nuclei
-PKKKRKV motif from the simian virus 40 large T antigen. When added to a eukaryotic protein, that protein localizes to the nucleus. in our hands, the localization appears to be very efficient (data coming soon). This tag is very handy for live cell fluorescent labeling, since image processing of glowing nuclei with nice defined edges is much simpler than quantifying diffuse signal in cell bodies with irregular edges.
+PKKKRKV motif from the Simian Virus 40 Large T-antigen. When added to a eukaryotic protein, that protein localizes to the nucleus. in our hands, the localization appears to be very efficient (data coming soon). This tag is very handy for live cell fluorescent labeling, since image processing of glowing nuclei with nice defined edges is much simpler than quantifying diffuse signal in cell bodies with irregular edges.
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