Difference between revisions of "Part:BBa J176133"

Revision as of 01:51, 11 December 2011

miRts BCL2

miR-34 target sequence, based on the BCL2 3' UTR Aliases: FC01

Usage and Biology

This part is intended to bring a synthetic gene under the control of miR-34 in human cells. A gene carrying a target site for a functional miR will be repressed by RNA interference. The target site works by placing it downstream of the ORF you want to repress. In our hands, a gene with this part does not appear to be repressed in one cell line (U2OS) where miR-34 is produced at a minimal level in proliferating cells. Perhaps this target requires higher levels of miR-34 to function.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 ===Usage and Biology===
-Insert this sequence into the 3' UTR of your gene construct (i.e., after the ORF) to place it under control of the natural microRNA miR-34 in human cells.
+This part is intended to bring a synthetic gene under the control of miR-34 in human cells. A gene carrying a target site for a functional miR will be repressed by RNA interference. The target site works by placing it downstream of the ORF you want to repress. In our hands, a gene with this part does not appear to be repressed in one cell line (U2OS) where miR-34 is produced at a minimal level in proliferating cells. Perhaps this target requires higher levels of miR-34 to function.
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