Difference between revisions of "Part:BBa M36306"
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_M36306 short</partinfo> | <partinfo>BBa_M36306 short</partinfo> | ||
Line 5: | Line 4: | ||
The coding sequence for the FseI restriction endonuclease codon optimized for use in E. coli. The enzyme will cleave DNA at sites with the sequence 5'...GGCCGG|CC...3'. The cutting position is indicated by '|' | The coding sequence for the FseI restriction endonuclease codon optimized for use in E. coli. The enzyme will cleave DNA at sites with the sequence 5'...GGCCGG|CC...3'. The cutting position is indicated by '|' | ||
− | Used to cut specific DNA sequences that FseI recognizes. | + | Used to cut specific DNA sequences that FseI recognizes. DOES NOT contain start codon. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 00:19, 9 December 2011
FseI restriction endonuclease
The coding sequence for the FseI restriction endonuclease codon optimized for use in E. coli. The enzyme will cleave DNA at sites with the sequence 5'...GGCCGG|CC...3'. The cutting position is indicated by '|'
Used to cut specific DNA sequences that FseI recognizes. DOES NOT contain start codon.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 436
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 499
Illegal PstI site found at 436 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 335
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 436
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 436
- 1000COMPATIBLE WITH RFC[1000]