Difference between revisions of "Part:BBa M36306"

 
 
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<partinfo>BBa_M36306 short</partinfo>
 
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The coding sequence for the FseI restriction endonuclease codon optimized for use in E. coli. The enzyme will cleave DNA at sites with the sequence 5'...GGCCGG|CC...3'. The cutting position is indicated by '|'
 
The coding sequence for the FseI restriction endonuclease codon optimized for use in E. coli. The enzyme will cleave DNA at sites with the sequence 5'...GGCCGG|CC...3'. The cutting position is indicated by '|'
  
Used to cut specific DNA sequences that FseI recognizes.
+
Used to cut specific DNA sequences that FseI recognizes. DOES NOT contain start codon.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:19, 9 December 2011

FseI restriction endonuclease

The coding sequence for the FseI restriction endonuclease codon optimized for use in E. coli. The enzyme will cleave DNA at sites with the sequence 5'...GGCCGG|CC...3'. The cutting position is indicated by '|'

Used to cut specific DNA sequences that FseI recognizes. DOES NOT contain start codon.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 499
    Illegal PstI site found at 436
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 335
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 436
  • 1000
    COMPATIBLE WITH RFC[1000]