Difference between revisions of "Part:BBa M36302:Design"
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Revision as of 00:07, 9 December 2011
Dual FseI restriction sites bounded by spacers
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal NgoMIV site found at 20 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This site was designed to guard against DNA methylation.
Source
References
FseI, a new type II restriction endonuclease that recognizes the octanucleotide sequence.
Nelson, J.M., Miceli, S.M., Lechevalier, M.P. and Robers, R.J. 1990. FseI, a new type II restriction
endonuclease that recognizes the octanucleotide sequence 5' GGCCGGCC 3'. Nucleic Acids Res., 18, 2061-2064.