Difference between revisions of "Part:BBa J176028:Design"
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* PCR-cloned from the pmirGLO plasmid (Promega) | * PCR-cloned from the pmirGLO plasmid (Promega) | ||
* Primers add XbaI upstream and SpeI/NotI/PstI downstream | * Primers add XbaI upstream and SpeI/NotI/PstI downstream | ||
| − | * | + | * A SpeI site and PstI site were mutated via site-directed mutagenesis (see sequence annotation) |
===Source=== | ===Source=== | ||
Latest revision as of 20:20, 5 April 2012
HPK
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 116
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- Created by Kevin Shee (undergraduate, Pam Silver's lab, 2010)
- PCR-cloned from the pmirGLO plasmid (Promega)
- Primers add XbaI upstream and SpeI/NotI/PstI downstream
- A SpeI site and PstI site were mutated via site-directed mutagenesis (see sequence annotation)
Source
- pmirGLO Dual-Luciferase miRNA Target expression vector (Promega)