Difference between revisions of "Part:BBa J176013"

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===Usage and Biology===
 
===Usage and Biology===
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[[Image:KAH_VP64.png|thumb|left|400px|'''VP64 Activation Domain Module''' VP64 is often fused to DNA binding domains to make synthetic transcription factors. Figure adapted from Beerli et al., 1998.]]
  
 
* Chassis: mammalian cells
 
* Chassis: mammalian cells

Revision as of 03:23, 22 November 2011

VP64

Tetrameric VP16 transcription activator domain

Usage and Biology

VP64 Activation Domain Module VP64 is often fused to DNA binding domains to make synthetic transcription factors. Figure adapted from Beerli et al., 1998.
  • Chassis: mammalian cells
  • Mammalian expression vector required
  • Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression
  • VP64 doesn't do much as a free-floating domain. Fuse it to a DNA binding domain


VP64 is a transcriptional activator composed of four tandem copies of VP16 (Herpes Simplex Viral Protein 16, amino acids 437-447, DALDDFDLDML) connected with glycine-serine linkers. When fused to another protein domain that can bind near the promoter of a gene, VP64 acts as a strong transcriptional activator. This module is a classic molecular biology tool, which pre-dates fancy terms like "synthetic biology." See the References section for some history on VP16 and VP64.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]