Difference between revisions of "Part:BBa J176013:Design"
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− | + | '''(1992) Seipel K, Georgiev O, Schaffner W. Different activation domains stimulate transcription from remote ('enhancer') and proximal ('promoter') positions. EMBO J. 1992 Dec;11(13):4961-8.'''<br> | |
− | Note: Earliest reported test of a "modular" VP16 "core" activator, DALDDFDLDML; activity was compared to activation domains from other transcription factors | + | Note: Earliest reported test of a "modular" VP16 "core" activator, DALDDFDLDML; activity was compared to activation domains from other transcription factors<br> |
− | + | ||
− | Note: VP16 tetramer VP64 ([DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]) fused to a zinc finger; synthetic construct added to NCBI database (GenBank: AAZ80416.1) in | + | '''(2005) Dreier B, Fuller RP, Segal DJ, Lund CV, Blancafort P, Huber A, Koksch B, Barbas CF 3rd. Development of Zinc Finger Domains for Recognition of the 5′-CNN-3′ Family DNA Sequences and Their Use in the Construction of Artificial Transcription Factors. J Biol Chem. 2005 Oct 21;280(42):35588-97.'''<br> |
+ | Note: VP16 tetramer VP64 ([DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]) fused to a zinc finger; synthetic construct added to NCBI database (GenBank: AAZ80416.1) in 2005 |
Revision as of 02:09, 22 November 2011
VP64
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
TBA
Source
TBA
References
In order of date published:
(1992) Seipel K, Georgiev O, Schaffner W. Different activation domains stimulate transcription from remote ('enhancer') and proximal ('promoter') positions. EMBO J. 1992 Dec;11(13):4961-8.
Note: Earliest reported test of a "modular" VP16 "core" activator, DALDDFDLDML; activity was compared to activation domains from other transcription factors
(2005) Dreier B, Fuller RP, Segal DJ, Lund CV, Blancafort P, Huber A, Koksch B, Barbas CF 3rd. Development of Zinc Finger Domains for Recognition of the 5′-CNN-3′ Family DNA Sequences and Their Use in the Construction of Artificial Transcription Factors. J Biol Chem. 2005 Oct 21;280(42):35588-97.
Note: VP16 tetramer VP64 ([DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]) fused to a zinc finger; synthetic construct added to NCBI database (GenBank: AAZ80416.1) in 2005