Difference between revisions of "Part:BBa K511822"

Latest revision as of 15:55, 5 November 2011

Inducible Yellow Fluorescent Protein Generator (UAS-Gal4-EYFP-FF4x4) MammoBlock Device

This MammoBlock composite device produces the yellow fluorescent protein EYFP-FF4x4 when induced with Gal4 transactivator variants and otherwise produces EYFP-FF4x4 at a low, basal (OFF) level. Note that the presence of the FF4 repeats on EYFP-FF4x4 means that this device can be repressed by the FF4 micro RNA.

Graph showing activation of the UAS_EYFP-FF4x4 by constitutively expressed Gal4-VP16.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 126
Illegal PstI site found at 447
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 126
Illegal NheI site found at 154
Illegal PstI site found at 447
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 126
Illegal XhoI site found at 83
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 126
Illegal PstI site found at 447
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 126
Illegal PstI site found at 447
1000
COMPATIBLE WITH RFC[1000]

Revision as of 15:55, 5 November 2011 (view source) Chsuqed (Talk \| contribs) ← Older edit		Latest revision as of 15:55, 5 November 2011 (view source) Chsuqed (Talk \| contribs)
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	Graph showing activation of the UAS_EYFP-FF4x4 by constitutively expressed Gal4-VP16.		Graph showing activation of the UAS_EYFP-FF4x4 by constitutively expressed Gal4-VP16.
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	https://static.igem.org/mediawiki/parts/2/2e/Uas_eyfp-4xff4_hef1a_gv16.jpg		https://static.igem.org/mediawiki/parts/2/2e/Uas_eyfp-4xff4_hef1a_gv16.jpg