Difference between revisions of "Part:BBa K649400"
 
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To characterize this part, we constructed the composite part, [https://parts.igem.org/Part:BBa_K649303 BBa_K649303]. BBa_K649303 is composed of PlacIQ(BBa_I14032) and RBS-''ispS''(BBa_K649400).<br>
 
To characterize this part, we constructed the composite part, [https://parts.igem.org/Part:BBa_K649303 BBa_K649303]. BBa_K649303 is composed of PlacIQ(BBa_I14032) and RBS-''ispS''(BBa_K649400).<br>
  
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[[Image:ispS_assay_result3.png|thumb|left|400px|isoprene synthesized by ''E. coli'' introduced ''ispS'' gene.<br>This work is done by Yuto Sugiuchi.]]
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[[Image:Rain-fig4-2.jpg|thumb|left|400px|isoprene synthesized by ''E. coli'' introduced ''ispS'' gene.<br>This work is done by Yuto Sugiuchi.]]
 
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Latest revision as of 05:17, 29 October 2011

isoprene synthase (ispS)

This part contains RBS and the isoprene synthase encoding gene(ispS). The ispS catalyzes the conversion of dimethylallyl diphoshate to isoprene.
To characterize this part, we constructed the composite part, BBa_K649303. BBa_K649303 is composed of PlacIQ(BBa_I14032) and RBS-ispS(BBa_K649400).

isoprene synthesized by E. coli introduced ispS gene.
This work is done by Yuto Sugiuchi.

If you are interested in the project related to this parts, please see [http://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/index.htm our project page in Tokyo_Tech 2011 wiki].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 762
    Illegal BglII site found at 1317
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]