Difference between revisions of "Part:BBa K567023"

Revision as of 03:25, 29 October 2011

PT7-Luc-4AGG-tRNA(Arg)

This part is constructed based on PT7-Luc-4AGG (BBa_K567009). The tDNA^Arg and luciferase gene into T7 operon so that both genes can be transcribed. tRNA^Arg can be correctly processed and become matured. Luciferase mRNA will be further translated into protein. The induced tRNA^Arg can facilitate the translation of luciferase-4AGG, achieving positive feedback. The transcription of this part is under the control of T7 promoter and lac operator. RNA expression is induced by 0.5mM IPTG when the OD600 of the culture reaches 0.3.

The working curve is shown here.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 48
Illegal PstI site found at 1929
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1929
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 48
Illegal PstI site found at 1929
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 48
Illegal PstI site found at 1929
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 917

@@ Line 4: / Line 4: @@
 This part is constructed based on PT7-Luc-4AGG ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K567009 BBa_K567009]). The tDNA<sup>Arg</sup> and luciferase gene into T7 operon so that both genes can be transcribed. tRNA<sup>Arg</sup> can be correctly processed and become matured. Luciferase mRNA will be further translated into protein. The induced tRNA<sup>Arg</sup> can facilitate the translation of luciferase-4AGG, achieving positive feedback.
 The transcription of this part is under the control of T7 promoter and ''lac'' operator. RNA expression is induced by 0.5mM IPTG when the OD600 of the culture reaches 0.3.
+The working curve is shown here.
+[[image:11SJTU_FB4.jpg|center|]]
 <!-- Add more about the biology of this part here