Difference between revisions of "Part:BBa K640007:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K640007 short</partinfo>
 
<partinfo>BBa_K640007 short</partinfo>
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===Design Notes===
 
===Design Notes===
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<3
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Both genes for the subparts were obtained from the <i>chlamydomonas</i> database.  Prefix and suffix were added through PCR.
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===Source===
 
===Source===
  
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<3
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http://www.chlamy.org/chlamydb.html
  
 
===References===
 
===References===
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Fuhrmann et al. (2004)
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  Plant Mol. Biol. 55, 869-881
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Heitzer and Zschoernig (2007)
 +
  Biotechniques 43, 324

Latest revision as of 22:58, 29 October 2011

Codon optimized Luciferase for chlamydomonas with Hsp70A promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 252
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1213


Design Notes

Both genes for the subparts were obtained from the chlamydomonas database. Prefix and suffix were added through PCR.


Source

http://www.chlamy.org/chlamydb.html

References

Fuhrmann et al. (2004) 

  Plant Mol. Biol. 55, 869-881

Heitzer and Zschoernig (2007) 

  Biotechniques 43, 324