Difference between revisions of "Part:BBa K649402"
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[[Image:RocF_and_Arg_box_on_3K3.png|thumb|center|500px|Urea concentration detected in bacterial samples on pSB3K3<br /> | [[Image:RocF_and_Arg_box_on_3K3.png|thumb|center|500px|Urea concentration detected in bacterial samples on pSB3K3<br /> | ||
| − | This work | + | This work was done by Natsuki Kubo.]] |
Introduction of [https://parts.igem.org/Part:BBa_K649402 BBa_K649402] containing arginase coding gene (<span style="font-style:italic;">rocF</span>) and Arg Box (an <span style="font-style:italic;">arg</span> operator capable of binding the arginine repressor) led to production of some urea in strain MG1655 (<span style="font-style:italic;">argR</span> +) and strain JE6852 (<span style="font-style:italic;">argR</span> -). But in both strains, the amount of urea produced was not much different from that when [https://parts.igem.org/Part:BBa_K649301 BBa_K649301] containing <span style="font-style:italic;">rocF</span> but not Arg box was introduced. This result shows that <span style="font-style:italic;">rocF</span> is working but Arg box is not working efficiently in this part. The reason why Arg box is not working is probably that low-copy-number plasmid used here is not capable of introducing enough number of Arg boxes to effectively deactivate the arginine repressor.<br /><br /> | Introduction of [https://parts.igem.org/Part:BBa_K649402 BBa_K649402] containing arginase coding gene (<span style="font-style:italic;">rocF</span>) and Arg Box (an <span style="font-style:italic;">arg</span> operator capable of binding the arginine repressor) led to production of some urea in strain MG1655 (<span style="font-style:italic;">argR</span> +) and strain JE6852 (<span style="font-style:italic;">argR</span> -). But in both strains, the amount of urea produced was not much different from that when [https://parts.igem.org/Part:BBa_K649301 BBa_K649301] containing <span style="font-style:italic;">rocF</span> but not Arg box was introduced. This result shows that <span style="font-style:italic;">rocF</span> is working but Arg box is not working efficiently in this part. The reason why Arg box is not working is probably that low-copy-number plasmid used here is not capable of introducing enough number of Arg boxes to effectively deactivate the arginine repressor.<br /><br /> | ||
Latest revision as of 05:30, 29 October 2011
Ptrc-RBS-rocF-Arg box
This part contains Ptrc & RBS & rocF & Arg box.
Introduction of BBa_K649402 containing arginase coding gene (rocF) and Arg Box (an arg operator capable of binding the arginine repressor) led to production of some urea in strain MG1655 (argR +) and strain JE6852 (argR -). But in both strains, the amount of urea produced was not much different from that when BBa_K649301 containing rocF but not Arg box was introduced. This result shows that rocF is working but Arg box is not working efficiently in this part. The reason why Arg box is not working is probably that low-copy-number plasmid used here is not capable of introducing enough number of Arg boxes to effectively deactivate the arginine repressor.
For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/data#2. our work in Tokyo_Tech 2011 wiki.]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 230
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]