Difference between revisions of "Part:BBa K523024"
 
Line 7: Line 7:
 
* A (possibly inactivated) cellulose binding domain
 
* A (possibly inactivated) cellulose binding domain
 
* The GH6 endoglucanase domain
 
* The GH6 endoglucanase domain
 +
 +
<font color="red">Edinburgh 2011 carried out some experiments on this part under the control of the lac promoter - see details at part <partinfo>BBa_K523025</partinfo>.</font>
  
  

Latest revision as of 18:36, 28 October 2011

cex + cenA fusion (cxnA)

Cex exoglucanase from Cellulomonas fimi fused to CenA endoglucanase from the same species. The part contains, in order:

  • The GH10 exoglucanase domain
  • A (possibly inactivated) cellulose binding domain
  • The GH6 endoglucanase domain

Edinburgh 2011 carried out some experiments on this part under the control of the lac promoter - see details at part BBa_K523025.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 524
    Illegal NotI site found at 2222
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2135
    Illegal XhoI site found at 1633
    Illegal XhoI site found at 1882
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
    Illegal NgoMIV site found at 530
    Illegal NgoMIV site found at 1032
    Illegal NgoMIV site found at 1413
    Illegal NgoMIV site found at 2338
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 577
    Illegal SapI.rc site found at 660